Wednesday, October 22, 2008

Workshop #6

6B) 
  • diffuse ions out of the cell
  • uni-directional transporter wants equilibrium in order to maintain cell's volume 
6C) 
  • phosphoenolpyruvate has the highest - delta G value
  • an enzyme with binding sites for ADP and Pe-P
  • radio label phosphate and filtrate
  • enzymes work both ways depending on concentration
6D) 
  1. add radio labeled glucose into the cell without ATP  
  2. wait
  3. filtrate and see if glucse is present:
-if gluscose is present, passive
-if glucose is not present, active
6F) 
  • Change polymerase binding sites from RNA to DNA
6G)
  • Double stranded DNA needs more energy
6H)
  • heating breaks H-bonds between bases
  • cooling reforms the double strands
6I)
  • its easier to find the codes

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